Hotspot

psbC-trnS-UGA

LSC · 36930-37056 · 127 bp

Back to Narcissus

Pi 0.0457

MarkerSeek score 66.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 13
pi: 0.0457
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCTAAGGGGTCCCAATGGTT	ACCATTTGATCCCTCGCTTC	50.0	50.0	57.9	58.0	827-839 bp	1.0	82.7
psbC-trnS-UGA_p2	TCTAAGGGGTCCCAATGGTT	TGGATCGGGAAAATGCCAAA	50.0	45.0	57.9	58.1	1077-1089 bp	1.0	82.0
psbC-trnS-UGA_p3	TCTAAGGGGTCCCAATGGTT	TTGGATCGGGAAAATGCCAA	50.0	45.0	57.9	58.1	1078-1090 bp	1.0	82.0
psbC-trnS-UGA_p4	TCTAAGGGGTCCCAATGGTT	TTTGGATCGGGAAAATGCCA	50.0	45.0	57.9	58.1	1079-1091 bp	1.0	82.0
psbC-trnS-UGA_p5	TCTAAGGGGTCCCAATGGTT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	517-519 bp	1.0	81.8