Hotspot

trnT-GGU-psbD

LSC · 33414-34498 · 1085 bp

Back to Narcissus

Pi 0.0184

MarkerSeek score 66.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 52
pi: 0.0184
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GCCTACAAAAACGAAACGGT	55.0	45.0	58.1	57.2	1224-1245 bp	1.0	78.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GCCTACAAAAACGAAACGGT	50.0	45.0	58.1	57.2	1227-1248 bp	1.0	78.4
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GCCTACAAAAACGAAACGGT	50.0	45.0	58.3	57.2	1201-1222 bp	1.0	77.0
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AGCCTACAAAAACGAAACGG	55.0	45.0	58.1	56.9	1225-1246 bp	1.0	76.3
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	AGCCTACAAAAACGAAACGG	50.0	45.0	58.1	56.9	1228-1249 bp	1.0	76.1