Hotspot

rpoB-trnC-GCA

LSC · 27554-28798 · 1245 bp

Back to Narcissus

Pi 0.0237

MarkerSeek score 71.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 73
pi: 0.0237
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1283-1313 bp	1.0	79.5
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1284-1314 bp	1.0	79.3
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.4	58.8	1320-1350 bp	1.0	77.4
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TTTGTTGATCAGGCGACACC	50.0	50.0	57.6	58.8	1321-1351 bp	1.0	77.2
rpoB-trnC-GCA_p5	TCCCTCATTTCCATTCCGGA	GACACCCGGATTTGAACTGG	50.0	55.0	58.4	58.8	1306-1336 bp	1.0	76.9