Hotspot

trnS-GCU-trnG-GCC

LSC · 8571-9834 · 1264 bp

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Pi 0.0416

MarkerSeek score 83.60

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 122
pi: 0.0416
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	45.0	40.9	58.0	57.3	1237-1341 bp	1.0	69.5
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	45.0	40.9	58.0	57.0	1238-1342 bp	1.0	67.5
trnS-GCU-trnG-GCC_p3	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1223-1327 bp	1.0	66.6
trnS-GCU-trnG-GCC_p4	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1218-1322 bp	1.0	65.8
trnS-GCU-trnG-GCC_p5	GATTCGAACCCTCGGTACAA	CGAACGAATCACACTTTTACCA	50.0	40.9	57.3	57.3	1273-1377 bp	1.0	64.3