primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	20	22	45.0	40.9	58.0	57.3	0.0	0.0	2.757064	8571	9834	1237-1341	1.000000	69.5
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	20	22	45.0	40.9	58.0	57.0	0.0	0.0	3.015948	8571	9834	1238-1342	1.000000	67.5
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	57.6	57.3	0.9	0.0	3.128806	8571	9834	1223-1327	1.000000	66.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	20	22	55.0	40.9	58.5	57.3	0.0	0.0	3.234820	8571	9834	1218-1322	1.000000	65.8
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	GATTCGAACCCTCGGTACAA	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	57.3	57.3	0.0	0.0	3.379046	8571	9834	1273-1377	1.000000	64.3
atpF_p1	atpF	1	CCGAGATTCAGAAGCGGAAA	GGGCTGATTTGAGGATGAGG	20	20	50.0	55.0	58.0	58.0	13.8	0.0	0.031691	12522	14026	2223-2319	1.000000	87.1
atpF_p2	atpF	2	CCGAGATTCAGAAGCGGAAA	CTCGTTGCCATTTGCTTCTG	20	20	50.0	50.0	58.0	58.0	13.8	0.0	0.037007	12522	14026	2303-2400	1.000000	87.1
atpF_p3	atpF	3	CCGAGATTCAGAAGCGGAAA	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	58.0	58.0	13.8	0.0	0.048532	12522	14026	2427-2524	1.000000	87.1
atpF_p4	atpF	4	AAATAAGCCTCGCTCACTGG	GGGCTGATTTGAGGATGAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051384	12522	14026	2153-2249	0.857143	81.2
atpF_p5	atpF	5	CCAAATAAGCCTCGCTCACT	GGGCTGATTTGAGGATGAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051384	12522	14026	2155-2251	0.857143	81.2
atpF-atpH_p1	atpF-atpH	1	CATATTGTGCCCGGACTTCA	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.075344	14027	14522	1041-1122	0.857143	82.8
atpF-atpH_p2	atpF-atpH	2	CATATTGTGCCCGGACTTCA	AAGCCGTAGAAGGTATTGCG	20	20	50.0	50.0	58.0	58.1	0.7	0.0	0.101025	14027	14522	1133-1214	0.857143	82.2
atpF-atpH_p3	atpF-atpH	3	CATATTGTGCCCGGACTTCA	CAATATAAAATGCGCGCGGT	20	20	50.0	45.0	58.0	58.0	0.7	33.1	0.067994	14027	14522	1458-1555	0.857143	81.7
atpF-atpH_p4	atpF-atpH	4	CATATTGTGCCCGGACTTCA	CCAATTGAGGCAATGCATGG	20	20	50.0	50.0	58.0	58.1	0.7	7.8	0.088853	14027	14522	1685-1800	0.857143	81.6
atpF-atpH_p5	atpF-atpH	5	CATATTGTGCCCGGACTTCA	CCCAATTGAGGCAATGCATG	20	20	50.0	50.0	58.0	58.1	0.7	7.8	0.088853	14027	14522	1686-1801	0.857143	81.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	57.5	0.0	14.6	0.934514	27554	28798	1283-1313	1.000000	79.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.6	57.5	0.0	14.6	0.960477	27554	28798	1284-1314	1.000000	79.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.4	58.8	0.0	0.0	1.174588	27554	28798	1320-1350	1.000000	77.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATTCCGG	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	57.6	58.8	0.0	0.0	1.200551	27554	28798	1321-1351	1.000000	77.2
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCCCTCATTTCCATTCCGGA	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	58.4	58.8	0.0	0.7	1.244140	27554	28798	1306-1336	1.000000	76.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGGGACTGCAAATCCTTTA	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	57.5	57.8	14.7	0.0	0.716233	28870	29930	1100-1146	1.000000	82.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	CCAGTTCAAATCCGGGTGTC	CAGCCCAAGCAAGACTTACT	20	20	55.0	50.0	58.8	57.8	0.0	0.0	1.025858	28870	29930	1077-1123	1.000000	80.2
trnC-GCA-petN_p3	trnC-GCA-petN	3	TATCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	57.0	57.8	0.0	0.0	1.212353	28870	29930	1082-1128	1.000000	78.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	AATTTATTTGGCGGCATGGC	CAGCCCAAGCAAGACTTACT	20	20	45.0	50.0	58.0	57.8	14.3	0.0	0.238935	28870	29930	1169-1169	0.142857	43.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	AATTTATTTGGCGGCATGGC	GCAGCCCAAGCAAGACTTA	20	19	45.0	52.6	58.0	58.1	14.3	0.0	1.100861	28870	29930	1170-1170	0.142857	36.9
petN-psbM_p1	petN-psbM	1	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAAATGA	20	20	55.0	35.0	58.1	53.9	0.0	0.0	4.237493	30021	30880	909-1019	1.000000	52.8
petN-psbM_p2	petN-psbM	2	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAAATGA	20	20	55.0	35.0	58.1	53.9	0.0	0.0	4.237493	30021	30880	908-1018	1.000000	52.8
petN-psbM_p3	petN-psbM	3	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAAATGA	20	20	55.0	35.0	57.8	53.9	0.0	0.0	4.294681	30021	30880	907-1017	1.000000	52.4
petN-psbM_p4	petN-psbM	4	AGTATGGGGAAGGAGTGGAC	TGTAAAAACAGTCAGCCAAAATGA	20	24	55.0	33.3	58.1	57.8	0.0	0.0	4.333250	30021	30880	913-1023	1.000000	52.0
petN-psbM_p5	petN-psbM	5	GTATGGGGAAGGAGTGGACT	TGTAAAAACAGTCAGCCAAAATGA	20	24	55.0	33.3	58.1	57.8	0.0	0.0	4.333250	30021	30880	912-1022	1.000000	52.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GCCTACAAAAACGAAACGGT	20	20	55.0	45.0	58.1	57.2	7.6	0.0	0.825460	33414	34498	1224-1245	1.000000	78.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GCCTACAAAAACGAAACGGT	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.847614	33414	34498	1227-1248	1.000000	78.4
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	GCCTACAAAAACGAAACGGT	20	20	50.0	45.0	58.3	57.2	0.0	0.0	1.044199	33414	34498	1201-1222	1.000000	77.0
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	AGCCTACAAAAACGAAACGG	20	20	55.0	45.0	58.1	56.9	7.6	0.0	1.115020	33414	34498	1225-1246	1.000000	76.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	AGCCTACAAAAACGAAACGG	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.137175	33414	34498	1228-1249	1.000000	76.1
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	TCTAAGGGGTCCCAATGGTT	ACCATTTGATCCCTCGCTTC	20	20	50.0	50.0	57.9	58.0	11.0	0.0	0.112954	36930	37056	827-839	1.000000	82.7
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	TCTAAGGGGTCCCAATGGTT	TGGATCGGGAAAATGCCAAA	20	20	50.0	45.0	57.9	58.1	11.0	0.0	0.138581	36930	37056	1077-1089	1.000000	82.0
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TCTAAGGGGTCCCAATGGTT	TTGGATCGGGAAAATGCCAA	20	20	50.0	45.0	57.9	58.1	11.0	0.0	0.138581	36930	37056	1078-1090	1.000000	82.0
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	TCTAAGGGGTCCCAATGGTT	TTTGGATCGGGAAAATGCCA	20	20	50.0	45.0	57.9	58.1	11.0	0.0	0.138581	36930	37056	1079-1091	1.000000	82.0
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TCTAAGGGGTCCCAATGGTT	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.9	58.1	11.0	0.0	0.127506	36930	37056	517-519	1.000000	81.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TAAAACCTGGCCTGTTGACC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.014917	48050	49050	1637-1704	1.000000	88.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGAAGCCATACCCAATCGAA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.056519	48050	49050	1694-1761	1.000000	88.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAAAACCTGGCCTGTTGACC	GATAGCCAGTTACCAACGCA	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.028836	48050	49050	1865-1932	1.000000	88.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CGAAGCCATACCCAATCGAA	GATAGCCAGTTACCAACGCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.070438	48050	49050	1922-1989	1.000000	87.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAAAACCTGGCCTGTTGACC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	3.3	0.0	0.079726	48050	49050	1645-1712	0.857143	81.1
atpB-rbcL_p1	atpB-rbcL	1	ACCGGAAGTAGTAGGATTGGT	AGTCTCTGTTTGTGGTGACA	21	20	47.6	45.0	57.9	56.6	9.5	0.0	2.483437	56954	57871	956-980	1.000000	68.5
atpB-rbcL_p2	atpB-rbcL	2	ACCGGAAGTAGTAGGATTGGT	AGTCTCTGTTTGTGGTGACAT	21	21	47.6	42.9	57.9	57.2	9.5	0.0	2.957522	56954	57871	956-980	1.000000	64.7
atpB-rbcL_p3	atpB-rbcL	3	ACCGGAAGTAGTAGGATTGGT	TGCTTTAGTCTCTGTTTGTGGT	21	22	47.6	40.9	57.9	57.8	9.5	0.0	3.262638	56954	57871	962-986	1.000000	62.3
atpB-rbcL_p4	atpB-rbcL	4	ACCGGAAGTAGTAGGATTGG	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	56.0	56.6	9.5	0.0	3.332103	56954	57871	956-980	1.000000	61.8
atpB-rbcL_p5	atpB-rbcL	5	CCGGAAGTAGTAGGATTGGT	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	56.0	56.6	0.0	0.0	3.332103	56954	57871	955-979	1.000000	61.8
rpl22-rps19_p1	rpl22-rps19	1	ACAAAAGGACCTCCGTTGAC	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.0	58.0	15.8	9.6	0.054216	86023	86076	367-372	0.857143	79.1
rpl22-rps19_p2	rpl22-rps19	2	CACAAAAGGACCTCCGTTGA	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.0	58.0	15.8	9.6	0.055594	86023	86076	368-373	0.857143	79.0
rpl22-rps19_p3	rpl22-rps19	3	TCACAAAAGGACCTCCGTTG	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.0	58.0	0.0	9.6	0.055594	86023	86076	369-374	0.857143	79.0
rpl22-rps19_p4	rpl22-rps19	4	ACAAAAGGACCTCCGTTGAC	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	15.8	22.4	0.061004	86023	86076	932-937	0.857143	76.2
rpl22-rps19_p5	rpl22-rps19	5	CACAAAAGGACCTCCGTTGA	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	15.8	22.4	0.062382	86023	86076	933-938	0.857143	76.2
ndhF_p1	ndhF	1	TATTGCAATTCCCCGAGTGG	TTTGTTCCCTTCGAAAGCGA	20	20	50.0	45.0	57.9	58.0	18.7	0.0	0.095038	114601	116814	2591-2612	0.571429	64.1
ndhF_p2	ndhF	2	GGAAGGGAAGCAGAACTAGC	TTTGTTCCCTTCGAAAGCGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.074962	114601	116814	2349-2358	0.428571	57.6
ndhF_p3	ndhF	3	GGAAGGGAAGCAGAACTAGC	CAGTTCCGCAATGAGTCAGA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.115868	114601	116814	2751-2764	0.428571	57.6
ndhF_p4	ndhF	4	GGAAGGGAAGCAGAACTAGC	TCAGTTCCGCAATGAGTCAG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.115868	114601	116814	2752-2765	0.428571	57.5
ndhF_p5	ndhF	5	AACTAGCTTTTGGGTCTGCC	TTTGTTCCCTTCGAAAGCGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.132646	114601	116814	2341-2341	0.142857	44.7
ndhF-rpl32_p1	ndhF-rpl32	1	ACTGGAAACGGAAGAAAAGGT	AGAAAAAGCTTTTATGGCAGC	21	21	42.9	38.1	57.7	55.6	0.0	24.1	4.647967	116815	117737	906-1075	1.000000	51.1
ndhF-rpl32_p2	ndhF-rpl32	2	ACTGGAAACGGAAGAAAAGGT	AAGAAAAAGCTTTTATGGCAGC	21	22	42.9	36.4	57.7	56.4	0.0	24.1	4.903293	116815	117737	907-1076	1.000000	49.1
ndhF-rpl32_p3	ndhF-rpl32	3	ACTGGAAACGGAAGAAAAGGT	GAAAAAGCTTTTATGGCAGC	21	20	42.9	40.0	57.7	54.1	0.0	24.1	5.215987	116815	117737	905-1074	1.000000	48.3
ndhF-rpl32_p4	ndhF-rpl32	4	ACTGGAAACGGAAGAAAAGGT	AAAGAAAAAGCTTTTATGGCAGC	21	23	42.9	34.8	57.7	57.1	0.0	24.1	5.222189	116815	117737	908-1077	1.000000	48.3
ndhF-rpl32_p5	ndhF-rpl32	5	ACTGGAAACGGAAGAAAAGGT	AGAAAAAGCTTTTATGGCAGCTA	21	23	42.9	34.8	57.7	57.0	0.0	24.1	5.275382	116815	117737	906-1075	1.000000	48.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACAAACAAGTAAGAAAATCTTGGA	TCCTAAGAGCAGCGTGTCTA	24	20	29.2	50.0	55.1	57.9	2.9	0.0	6.976329	117912	118696	768-860	1.000000	48.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACAAACAAGTAAGAAAATCTTGGA	AGCAGCGTGTCTACCAATTT	24	20	29.2	45.0	55.1	57.8	2.9	0.0	7.037056	117912	118696	761-853	1.000000	48.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACAAACAAGTAAGAAAATCTTGGA	CCTAAGAGCAGCGTGTCTAC	24	20	29.2	55.0	55.1	57.8	2.9	0.0	7.048347	117912	118696	767-859	1.000000	48.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACAAACAAGTAAGAAAATCTTGGA	CTAAGAGCAGCGTGTCTACC	24	20	29.2	55.0	55.1	57.8	2.9	0.0	7.048347	117912	118696	766-858	1.000000	48.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACAAACAAGTAAGAAAATCTTGGA	GAGCAGCGTGTCTACCAATT	24	20	29.2	50.0	55.1	58.3	2.9	0.0	7.125199	117912	118696	762-854	1.000000	48.4
ccsA-ndhD_p1	ccsA-ndhD	1	ACCTGCCCTACAATCTCAGT	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057448	119821	120013	1133-1133	1.000000	83.4
ccsA-ndhD_p2	ccsA-ndhD	2	ACCTGCCCTACAATCTCAGT	CTCGATGGCTTCTCTTGCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.090884	119821	120013	1131-1131	1.000000	83.1
ccsA-ndhD_p3	ccsA-ndhD	3	ACCTGCCCTACAATCTCAGT	TTCTTGGTGGGAACGAGTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.079918	119821	120013	1235-1235	1.000000	82.9
ccsA-ndhD_p4	ccsA-ndhD	4	TTTTACCCAAGGTTTCGCCA	TTCTCGATGGCTTCTCTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.084570	119821	120013	1196-1196	0.857143	77.1
ccsA-ndhD_p5	ccsA-ndhD	5	ACCTGCCCTACAATCTCAGT	TGTCCCTGGTTGGTGATAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080575	119821	120013	1445-1445	0.857143	76.5
ycf1_p1	ycf1	1	TTCGCCACTTTTTCGCTACT	TATTGCAATTCCCCGAGTGG	20	20	45.0	50.0	58.1	57.9	0.0	18.7	0.177637	130835	130954	1239-1347	0.857143	80.1
ycf1_p2	ycf1	2	TTCGCCACTTTTTCGCTACT	GGAAGGGAAGCAGAACTAGC	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.157561	130835	130954	985-994	0.571429	65.9
ycf1_p3	ycf1	3	AGTTGTGCATTGATCCTCGG	GGAAGGGAAGCAGAACTAGC	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.288497	130835	130954	629-638	0.571429	65.7
ycf1_p4	ycf1	4	TTCGCCACTTTTTCGCTACT	GTCTGAAACCTTGGCGAAGA	20	20	45.0	50.0	58.1	58.1	0.0	10.4	0.247349	130835	130954	1086-1095	0.571429	64.8
ycf1_p5	ycf1	5	TTCGCCACTTTTTCGCTACT	AACTAGCTTTTGGGTCTGCC	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.215245	130835	130954	981-981	0.142857	44.0