Hotspot

ycf1

SSC · 124538-130168 · 5631 bp

Back to Naravelia

Pi 0.00213
MarkerSeek score 53.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5631
variable_sites
12
pi
0.00213
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGAAATATTCCGTGTGTTCCA ACGGATAATTCCAATGATGAAACA 38.1 33.3 55.5 57.0 296 bp 1.0 41.1
ycf1_p2 TGAAATATTCCGTGTGTTCCAA ACGGATAATTCCAATGATGAAACA 36.4 33.3 56.3 57.0 296 bp 1.0 41.1
ycf1_p3 TGAAATATTCCGTGTGTTCCA AACGGATAATTCCAATGATGAAACA 38.1 32.0 55.5 57.6 297 bp 1.0 41.1
ycf1_p4 TGAAATATTCCGTGTGTTCCA ACGGATAATTCCAATGATGAAACAA 38.1 32.0 55.5 57.6 296 bp 1.0 41.1
ycf1_p5 ATGAAATATTCCGTGTGTTCCA ACGGATAATTCCAATGATGAAACA 36.4 33.3 56.1 57.0 297 bp 1.0 41.1