Hotspot

ndhE

SSC · 118220-118525 · 306 bp

Back to Naravelia

Pi 0

MarkerSeek score 25.20

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCTGCTCATGAAAGAAGGCC	TCGAGATTGATGAGGAGTTGT	50.0	42.9	57.9	56.5	447-448 bp	1.0	59.0
ndhE_p2	CTGCTCATGAAAGAAGGCCA	TCGAGATTGATGAGGAGTTGT	50.0	42.9	58.2	56.5	446-447 bp	1.0	58.6
ndhE_p3	TGCTCATGAAAGAAGGCCAA	TCGAGATTGATGAGGAGTTGT	45.0	42.9	57.7	56.5	445-446 bp	1.0	57.6
ndhE_p4	TCTGCTCATGAAAGAAGGCC	TCGAGATTGATGAGGAGTTGTT	50.0	40.9	57.9	57.2	447-448 bp	1.0	56.8
ndhE_p5	CTGCTCATGAAAGAAGGCCA	TCGAGATTGATGAGGAGTTGTT	50.0	40.9	58.2	57.2	446-447 bp	1.0	56.4