Hotspot

ndhA

SSC · 120443-122540 · 2098 bp

Back to Naravelia

Pi 0.00524

MarkerSeek score 60.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2098
variable_sites: 11
pi: 0.00524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGAATCCGGTTACCATGGG	TGGGAGAAGTTGATCGTTGA	50.0	45.0	57.3	56.5	2218 bp	1.0	63.0
ndhA_p2	AATCCGGTTACCATGGGGAA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.7	56.5	2215 bp	1.0	62.7
ndhA_p3	ATGAATCCGGTTACCATGGG	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	57.3	57.2	2218 bp	1.0	61.0
ndhA_p4	AATCCGGTTACCATGGGGAA	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	58.7	57.2	2215 bp	1.0	60.7
ndhA_p5	CGGTTACCATGGGGAACATA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	57.0	56.5	2211 bp	1.0	60.2