Hotspot

rpl32-trnL-UAG

SSC · 113744-114664 · 921 bp

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Pi 0.00543
MarkerSeek score 49.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
921
variable_sites
5
pi
0.00543
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCAAGTGTATTGGGCAGGAA AGCGTGTCTACCGATTTCAC 45.0 50.0 56.7 58.0 973 bp 1.0 70.2
rpl32-trnL-UAG_p2 TCAAGTGTATTGGGCAGGAA CAGCGTGTCTACCGATTTCA 45.0 50.0 56.7 58.0 974 bp 1.0 70.2
rpl32-trnL-UAG_p3 TCAAGTGTATTGGGCAGGAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 56.7 57.9 983 bp 1.0 69.3
rpl32-trnL-UAG_p4 TCAAGTGTATTGGGCAGGAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 56.7 57.8 982 bp 1.0 68.7
rpl32-trnL-UAG_p5 TCAAGTGTATTGGGCAGGAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 56.7 57.8 981 bp 1.0 68.7