Hotspot

psbE-petL

LSC · 64138-65477 · 1340 bp

Back to Naravelia

Pi 0.00376

MarkerSeek score 61.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1340
variable_sites: 5
pi: 0.00376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CCGCCAGTAGAAAACCGAAA	50.0	50.0	57.9	58.5	1484-1497 bp	1.0	76.5
psbE-petL_p2	ACGAATAACCAACCCGCAAT	CCGCCAGTAGAAAACCGAAA	45.0	50.0	58.2	58.5	1493-1506 bp	1.0	75.5
psbE-petL_p3	TGCTGACGAATAACCAACCC	CCGCCAGTAGAAAACCGAAA	50.0	50.0	58.2	58.5	1498-1511 bp	1.0	75.4
psbE-petL_p4	CGAATAACCAACCCGCAATG	CCGCCAGTAGAAAACCGAAA	50.0	50.0	57.8	58.5	1492-1505 bp	1.0	75.4
psbE-petL_p5	ACCAACCCGCAATGAATAGG	CCGCCAGTAGAAAACCGAAA	50.0	50.0	58.2	58.5	1486-1499 bp	1.0	75.0