Hotspot

rbcL-accD

LSC · 56646-57383 · 738 bp

Back to Naravelia

Pi 0.00271

MarkerSeek score 60.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 2
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	GGTCGAATACCCCCTAGTGA	50.0	55.0	57.9	57.9	887-888 bp	1.0	78.6
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ATCGAAGTGAACCGCCATTT	50.0	45.0	57.9	58.2	824-825 bp	1.0	77.9
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	ATTTACTTAACCCGCACCCG	50.0	50.0	57.9	58.3	868-869 bp	1.0	77.2
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	AGTGAACCGCCATTTTTCCA	50.0	45.0	57.9	58.3	819-820 bp	1.0	76.9
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	CACCCGTGTTCTAACTCCTC	50.0	55.0	57.9	57.6	854-855 bp	1.0	76.4