Hotspot

petN-psbM

LSC · 32436-33229 · 794 bp

Back to Naravelia

Pi 0.0115

MarkerSeek score 66.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 9
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGGGGGCATAATTCACATG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.0	57.6	972-980 bp	1.0	70.0
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	887-895 bp	1.0	67.5
petN-psbM_p3	AAGTCTTGCTTGGTCTGCTT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.6	57.6	944-952 bp	1.0	67.3
petN-psbM_p4	AGTCTTGCTTGGTCTGCTTT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.6	57.6	943-951 bp	1.0	67.3
petN-psbM_p5	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	970-978 bp	1.0	67.1