Hotspot

rpoB-trnC-GCA

LSC · 30746-31828 · 1083 bp

Back to Naravelia

Pi 0.0028

MarkerSeek score 61.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 3
pi: 0.0028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1159-1171 bp	1.0	75.1
rpoB-trnC-GCA_p2	GTTCTTCCGTCAATCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1236-1248 bp	1.0	74.2
rpoB-trnC-GCA_p3	AGTTCTTCCGTCAATCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1237-1249 bp	1.0	74.1
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	GCAAAGGGTTTCGGGTTTTT	50.0	45.0	57.3	57.7	1195-1207 bp	1.0	73.1
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCG	TTATTGATCAGGCGACACCC	50.0	50.0	57.3	57.7	1177-1189 bp	1.0	73.1