Hotspot

rps4-rps16

LSC · 634-1185 · 552 bp

Back to Naravelia

Pi 0.0164
MarkerSeek score 67.50
Primer available yes
Label rps4-rps16

Feature Metadata

label_name
rps4-rps16
feature_type
igs
region
LSC
length_bp
552
variable_sites
9
pi
0.0164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-rps16_p1 GTAAGTGGGTCGGTTTGGAA ATTCCTTCAAGTCGCACGTT 50.0 45.0 57.7 58.1 674-676 bp 1.0 78.3
rps4-rps16_p2 GTAAGTGGGTCGGTTTGGAA AAGTCGCACGTTGCTTTCTA 50.0 45.0 57.7 58.1 666-668 bp 1.0 78.1
rps4-rps16_p3 AAGTGGGTCGGTTTGGAAAT ATTCCTTCAAGTCGCACGTT 45.0 45.0 57.6 58.1 672-674 bp 1.0 77.3
rps4-rps16_p4 AAGTGGGTCGGTTTGGAAAT AAGTCGCACGTTGCTTTCTA 45.0 45.0 57.6 58.1 664-666 bp 1.0 77.2
rps4-rps16_p5 AGTAAGTGGGTCGGTTTGGA ATTCCTTCAAGTCGCACGTT 50.0 45.0 58.6 58.1 675-677 bp 1.0 75.7