Hotspot

rpl36-rps8

LSC · 74008-74394 · 387 bp

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Pi 0.0566

MarkerSeek score 73.70

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 21
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CCCTTAATGCCGCATCTCTT	AAATAGACCGGGACCAAAGC	50.0	50.0	58.0	57.9	1152-1159 bp	1.0	82.9
rpl36-rps8_p2	CCCTTAATGCCGCATCTCTT	ATTTTAGGCGGGATAGGGGT	50.0	50.0	58.0	58.2	1058-1065 bp	1.0	82.8
rpl36-rps8_p3	CCCTTAATGCCGCATCTCTT	TCGTTTCATTAGCGCCTGAA	50.0	45.0	58.0	57.9	1563-1571 bp	1.0	82.4
rpl36-rps8_p4	CCCTTAATGCCGCATCTCTT	CCCAAGAATTTTAGGCGGGA	50.0	50.0	58.0	57.9	1065 bp	0.5	58.7
rpl36-rps8_p5	CCCTTAATGCCGCATCTCTT	TCCCAAGAATTTTAGGCGGG	50.0	50.0	58.0	57.9	1066 bp	0.5	58.7