Hotspot

psbE-petL

LSC · 59456-60462 · 1007 bp

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Pi 0.0384

MarkerSeek score 70.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 31
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	945-1076 bp	1.0	71.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	940-1071 bp	1.0	71.2
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.4	56.3	938-1069 bp	1.0	70.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGTAGAAAACCGA	50.0	42.9	57.8	57.0	946-1077 bp	1.0	69.6
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGAA	50.0	42.9	57.8	57.0	945-1076 bp	1.0	69.6