Hotspot

trnV-UAC-trnM-CAU

LSC · 47430-47594 · 165 bp

Back to Nanocnide

Pi 0.051

MarkerSeek score 76.50

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 8
pi: 0.051
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	GCGTGTAAACAAGGTGCTCT	TTGAACCAATGACTCCTGCC	50.0	50.0	58.8	58.1	259-264 bp	1.0	78.0
trnV-UAC-trnM-CAU_p2	GCGTGTAAACAAGGTGCTCT	CTCCTGCCGTATGAAAGCAA	50.0	50.0	58.8	58.3	247-252 bp	1.0	76.8
trnV-UAC-trnM-CAU_p3	GCGTGTAAACAAGGTGCTCT	TCCTGCCGTATGAAAGCAAT	50.0	45.0	58.8	57.6	246-251 bp	1.0	75.6
trnV-UAC-trnM-CAU_p4	GCGTGTAAACAAGGTGCTCT	CCAATGACTCCTGCCGTATG	50.0	55.0	58.8	58.4	254-259 bp	1.0	75.5
trnV-UAC-trnM-CAU_p5	GCGTGTAAACAAGGTGCTCT	CAATGACTCCTGCCGTATGA	50.0	50.0	58.8	57.4	253-258 bp	1.0	74.0