Pi
0.0826
MarkerSeek score
79.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 557-590 bp | 1.0 | 88.1 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 565-598 bp | 1.0 | 87.5 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 571-604 bp | 1.0 | 86.1 |
| trnT-UGU-trnL-UAA_p4 | CTAACCTCTGAGCTAAGCGG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.5 | 58.0 | 549-582 bp | 1.0 | 85.2 |
| trnT-UGU-trnL-UAA_p5 | ATGCGATGCTCTAACCTCTG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.5 | 58.0 | 559-592 bp | 1.0 | 84.9 |