Hotspot

petN-psbM

LSC · 26138-26547 · 410 bp

Back to Nanocnide

Pi 0.0757

MarkerSeek score 74.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 28
pi: 0.0757
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AAAGGCCGGTTATGCGTATT	ACCAATGGTAGGGCACTTTG	45.0	50.0	58.0	58.1	1164 bp	0.5	59.0
petN-psbM_p2	AAGGCCGGTTATGCGTATTT	ACCAATGGTAGGGCACTTTG	45.0	50.0	58.0	58.1	1163 bp	0.5	59.0
petN-psbM_p3	AAGGCCGGTTATGCGTATTT	CAACCAATGGTAGGGCACTT	45.0	50.0	58.0	58.1	1165 bp	0.5	59.0
petN-psbM_p4	AGGCCGGTTATGCGTATTTT	ACCAATGGTAGGGCACTTTG	45.0	50.0	58.0	58.1	1162 bp	0.5	59.0
petN-psbM_p5	AGGCCGGTTATGCGTATTTT	CAACCAATGGTAGGGCACTT	45.0	50.0	58.0	58.1	1164 bp	0.5	59.0