Hotspot

trnK-UUU-trnQ-UUG

LSC · 4166-5398 · 1233 bp

Back to Nanocnide

Pi 0.0561
MarkerSeek score 74.40
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1233
variable_sites
58
pi
0.0561
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AGTCGCGGTCTTACAAACTC AGCAGCTTGCCAAACAAAAG 50.0 45.0 57.9 58.1 1807-1950 bp 1.0 86.3
trnK-UUU-trnQ-UUG_p2 GTCGCGGTCTTACAAACTCT AGCAGCTTGCCAAACAAAAG 50.0 45.0 57.9 58.1 1806-1949 bp 1.0 86.3
trnK-UUU-trnQ-UUG_p3 AGTCGCGGTCTTACAAACTC CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1812-1955 bp 1.0 86.2
trnK-UUU-trnQ-UUG_p4 GTCGCGGTCTTACAAACTCT CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1811-1954 bp 1.0 86.2
trnK-UUU-trnQ-UUG_p5 CCTTTCCCACATCAAGCACT AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.1 58.1 2097-2236 bp 1.0 85.9