Hotspot

ndhA

SSC · 115316-117439 · 2124 bp

Back to Nannoglottis

Pi 0.00282

MarkerSeek score 47.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2124
variable_sites: 6
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCATTATGGGAGAAGTTGATCGT	TGTTTGAGACAAGTCGTGAA	39.1	40.0	57.4	55.2	2187-2188 bp	1.0	40.2
ndhA_p2	TCATTATGGGAGAAGTTGATCGT	TTGTTTGAGACAAGTCGTGA	39.1	40.0	57.4	55.2	2188-2189 bp	1.0	40.2
ndhA_p3	TCATTATGGGAGAAGTTGATCGT	TTGTTTGAGACAAGTCGTGAA	39.1	38.1	57.4	56.0	2188-2189 bp	1.0	40.2
ndhA_p4	TGGGAGAAGTTGATCGTTAA	TGTTTGAGACAAGTCGTGAA	40.0	40.0	54.0	55.2	2181-2182 bp	1.0	40.2
ndhA_p5	TGGGAGAAGTTGATCGTTAA	TTGTTTGAGACAAGTCGTGA	40.0	40.0	54.0	55.2	2182-2183 bp	1.0	40.2