Hotspot

matK

LSC · 1865-3373 · 1509 bp

Back to Nannoglottis

Pi 0.00331

MarkerSeek score 60.60

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 5
pi: 0.00331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATACCACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	34.8	50.0	56.2	57.6	1655-1656 bp	1.0	40.2
matK_p2	TCCTTACTACATACCACATCAACA	ACCCCCAAAATCCTCTACCT	37.5	50.0	57.1	57.6	1663-1664 bp	1.0	40.2
matK_p3	ACCACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	35.0	50.0	53.0	57.6	1651-1652 bp	1.0	40.2
matK_p4	ACATACCACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	33.3	50.0	56.9	57.6	1655-1656 bp	1.0	40.2
matK_p5	CCTTACTACATACCACATCAACA	ACCCCCAAAATCCTCTACCT	39.1	50.0	55.8	57.6	1662-1663 bp	1.0	40.2