Hotspot

rpl32-ndhF

SSC · 124060-125120 · 1061 bp

Back to Nannoglottis

Pi 0.00754

MarkerSeek score 62.70

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1061
variable_sites: 8
pi: 0.00754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACGACGCCCAATATCCTTTC	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	1244 bp	1.0	79.9
rpl32-ndhF_p2	ACGACGCCCAATATCCTTTC	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	1242 bp	1.0	79.8
rpl32-ndhF_p3	ACGACGCCCAATATCCTTTC	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	58.1	58.0	1243 bp	1.0	79.8
rpl32-ndhF_p4	ACGACGCCCAATATCCTTTC	CTTTTTGTTGCCGTCGGAAA	50.0	45.0	58.1	57.8	1250 bp	1.0	78.5
rpl32-ndhF_p5	ACGACGCCCAATATCCTTTC	TTTGTTGCCGTCGGAAAAAG	50.0	45.0	58.1	57.8	1247 bp	1.0	78.5