Hotspot

petL-petG

LSC · 65592-65756 · 165 bp

Back to Nannoglottis

Pi 0

MarkerSeek score 27.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TAGCTCCTTGATGCCTACCA	TCAAAGGTCCAACTGATCGC	50.0	50.0	57.8	58.2	385 bp	1.0	77.1
petL-petG_p2	AGCTCCTTGATGCCTACCAT	TCAAAGGTCCAACTGATCGC	50.0	50.0	58.5	58.2	384 bp	1.0	74.6
petL-petG_p3	TAGCTCCTTGATGCCTACCA	CGCCACGCCTGTATTGTAAA	50.0	50.0	57.8	58.9	368 bp	1.0	71.2
petL-petG_p4	TGGTCTGAGCAAGATACGAC	TCAAAGGTCCAACTGATCGC	50.0	50.0	57.1	58.2	307 bp	1.0	70.9
petL-petG_p5	AGCTCCTTGATGCCTACCA	TCAAAGGTCCAACTGATCGC	52.6	50.0	58.0	58.2	384 bp	1.0	70.4