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petA-psbJ

LSC · 62739-63458 · 720 bp

Back to Nannoglottis

Pi 0.00694

MarkerSeek score 62.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 5
pi: 0.00694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	858-862 bp	1.0	77.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	848-852 bp	1.0	77.1
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	896-900 bp	1.0	75.5
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	895-899 bp	1.0	75.5
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	57.9	57.4	886-890 bp	1.0	75.5