Hotspot

accD-psaI

LSC · 58192-58941 · 750 bp

Back to Nannoglottis

Pi 0.00267

MarkerSeek score 60.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 2
pi: 0.00267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	931-932 bp	1.0	66.1
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	930-931 bp	1.0	66.1
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	932-933 bp	1.0	66.1
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.0	58.0	931-932 bp	1.0	64.2
accD-psaI_p5	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	921-922 bp	1.0	64.1