Hotspot

ycf34

Genome · 34784-35035 · 252 bp

Back to Nannochloropsis

Pi 0.124

MarkerSeek score 72.60

Primer available yes

Label ycf34

Feature Metadata

label_name: ycf34
feature_type: gene
region: Genome
length_bp: 252
variable_sites: 57
pi: 0.124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf34_p1	GGACTGAAGATCCGTGTGTC	CTGTTGCGTTTGCCCTATTG	55.0	50.0	58.0	58.0	935-966 bp	1.0	91.8
ycf34_p2	GCAATGGACTGAAGATCCGT	CTGTTGCGTTTGCCCTATTG	50.0	50.0	58.0	58.0	940-971 bp	1.0	91.6
ycf34_p3	GGACTGAAGATCCGTGTGTC	CCCATACTGTTTGCAGGTGT	55.0	50.0	58.0	58.1	1174-1205 bp	1.0	90.5
ycf34_p4	GGACTGAAGATCCGTGTGTC	TGCACCGTGTAATGCGATAA	55.0	45.0	58.0	58.0	1274-1305 bp	0.75	80.6
ycf34_p5	GCAATGGACTGAAGATCCGT	TGCACCGTGTAATGCGATAA	50.0	45.0	58.0	58.0	1279-1310 bp	0.75	80.3