Hotspot

rpoA

Genome · 20330-21652 · 1323 bp

Back to Nannochloropsis

Pi 0.0707

MarkerSeek score 79.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1323
variable_sites: 172
pi: 0.0707
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CCCACCACCAGAAACATCAA	ACATAATGGGTGTCGTGCTC	50.0	50.0	58.0	58.0	2136 bp	0.75	75.9
rpoA_p2	CCCACCACCAGAAACATCAA	AAATGGACCTGGAAACGGTC	50.0	50.0	58.0	58.1	2229 bp	0.5	63.7
rpoA_p3	CCCACCACCAGAAACATCAA	CGACGTCACATACAGGGTTT	50.0	50.0	58.0	57.9	2657 bp	0.5	63.6
rpoA_p4	CCCACCACCAGAAACATCAA	GGCAGGAAGTAGCGGTTTTA	50.0	50.0	58.0	57.9	2349 bp	0.5	63.6
rpoA_p5	CCCACCACCAGAAACATCAA	ACGGTCGTGAAATTGCCATA	50.0	45.0	58.0	57.9	2215 bp	0.5	61.8