Hotspot

clpC-I

Genome · 46004-47347 · 1344 bp

Back to Nannochloropsis

Pi 0.105

MarkerSeek score 72.60

Primer available yes

Label clpC-I

Feature Metadata

label_name: clpC-I
feature_type: gene
region: Genome
length_bp: 1344
variable_sites: 247
pi: 0.105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC-I_p1	CCACCACCCCATTTTTCTGA	GTTTCCATCCGTTATCCCCC	50.0	55.0	58.0	58.0	2262-2295 bp	1.0	89.3
clpC-I_p2	CCACCACCCCATTTTTCTGA	TTCTCTTCAGGCAGAGGTGA	50.0	50.0	58.0	58.0	2363-2371 bp	0.75	76.6
clpC-I_p3	AGGGCAAAACGAGGTTTGAT	TTCTCTTCAGGCAGAGGTGA	45.0	50.0	58.0	58.0	1930 bp	0.5	59.9
clpC-I_p4	AGGGCAAAACGAGGTTTGAT	GTTTCCATCCGTTATCCCCC	45.0	55.0	58.0	58.0	1854 bp	0.5	59.8
clpC-I_p5	AGGGCAAAACGAGGTTTGAT	GGCATATGGGGTTTTAGCGA	45.0	50.0	58.0	58.0	1876 bp	0.5	59.8