Hotspot

chlI

Genome · 37972-39207 · 1236 bp

Back to Nannochloropsis

Pi 0.0612

MarkerSeek score 79.30

Primer available yes

Label chlI

Feature Metadata

label_name: chlI
feature_type: gene
region: Genome
length_bp: 1236
variable_sites: 138
pi: 0.0612
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI_p1	CCATGGGTAGAACCACTTCG	CGGTGATTTCTTTGCAGCTG	55.0	50.0	58.0	58.0	2350-2354 bp	0.75	76.1
chlI_p2	TGGGATTTACGTGCTCCATG	CGGTGATTTCTTTGCAGCTG	50.0	50.0	58.0	58.0	2365-2369 bp	0.75	75.9
chlI_p3	GATTTACGTGCTCCATGGGT	CGGTGATTTCTTTGCAGCTG	50.0	50.0	58.0	58.0	2362-2366 bp	0.75	75.9
chlI_p4	CCATGGGTAGAACCACTTCG	GTGCCAAGATGAAAATCGCC	55.0	50.0	58.0	58.1	2372-2381 bp	0.75	75.4
chlI_p5	CCATGGGTAGAACCACTTCG	GCCAAGATGAAAATCGCCAC	55.0	50.0	58.0	58.1	2370-2373 bp	0.5	62.6