Hotspot

chlB

Genome · 41249-42769 · 1521 bp

Back to Nannochloropsis

Pi 0.0602

MarkerSeek score 79.40

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 1521
variable_sites: 166
pi: 0.0602
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	AGACGTCTAGTGGAACGACT	ACACGCGCCTAGTTTTTACA	50.0	45.0	57.8	58.1	2325-2327 bp	1.0	85.6
chlB_p2	AGACGTCTAGTGGAACGACT	GCCACAAATTGCTCGTGATC	50.0	50.0	57.8	58.1	2193-2194 bp	0.75	74.5
chlB_p3	CCGATTGCTCTACCACTGAG	GCCACAAATTGCTCGTGATC	55.0	50.0	57.8	58.1	2435-2436 bp	0.75	74.5
chlB_p4	AGACGTCTAGTGGAACGACT	AAAGCCACAAATTGCTCGTG	50.0	45.0	57.8	57.9	2196-2197 bp	0.75	74.2
chlB_p5	AGACGTCTAGTGGAACGACT	ATTAGGCTCCGTGCTATTGC	50.0	50.0	57.8	58.1	2039-2040 bp	0.5	61.1