Hotspot

rps16-P417_t022

Genome · 99715-100553 · 839 bp

Back to Nannochloropsis

Pi 0.0747
MarkerSeek score 80.20
Primer available yes
Label rps16-P417_t022

Feature Metadata

label_name
rps16-P417_t022
feature_type
igs
region
Genome
length_bp
839
variable_sites
113
pi
0.0747
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-P417_t022_p1 TCCAAAAGCAATTCCACCGA TATCGCCAGGTTGACATTGG 45.0 50.0 58.0 58.0 1950-1953 bp 1.0 87.8
rps16-P417_t022_p2 CTACCCCATACCACCATTGC TATCGCCAGGTTGACATTGG 55.0 50.0 58.0 58.0 1875-1878 bp 1.0 87.8
rps16-P417_t022_p3 GAGCACCTTGTGATAAGCGA TATCGCCAGGTTGACATTGG 50.0 50.0 58.0 58.0 1637-1640 bp 0.5 64.3
rps16-P417_t022_p4 GAGCACCTTGTGATAAGCGA AGTTGAATGCTATCGCCAGG 50.0 50.0 58.0 58.0 1647-1650 bp 0.5 64.2
rps16-P417_t022_p5 TCCAAAAGCAATTCCACCGA AGTTGAATGCTATCGCCAGG 45.0 50.0 58.0 58.0 1960-1963 bp 0.5 63.9