Hotspot

rbcS-psbA

Genome · 92991-93369 · 379 bp

Back to Nannochloropsis

Pi 0.231

MarkerSeek score 80.30

Primer available yes

Label rbcS-psbA

Feature Metadata

label_name: rbcS-psbA
feature_type: igs
region: Genome
length_bp: 379
variable_sites: 139
pi: 0.231
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcS-psbA_p1	TCCTCCTGGAGAACGTTACG	GTTGCATTAAACGCTCCTGC	55.0	50.0	58.8	58.4	423-760 bp	1.0	82.3
rbcS-psbA_p2	TCCTCCTGGAGAACGTTACG	TTGCATTAAACGCTCCTGCT	55.0	45.0	58.8	58.5	422-759 bp	1.0	81.6
rbcS-psbA_p3	CCTCCTGGAGAACGTTACGT	GTTGCATTAAACGCTCCTGC	55.0	50.0	59.1	58.4	422-759 bp	1.0	80.0
rbcS-psbA_p4	CCTCCTGGAGAACGTTACGT	TTGCATTAAACGCTCCTGCT	55.0	45.0	59.1	58.5	421-758 bp	1.0	79.4
rbcS-psbA_p5	TCCTCCTGGAGAACGTTACG	CGCTCCTGCTGTTAATGCTT	55.0	50.0	58.8	58.9	412-749 bp	1.0	78.1