Hotspot

thiG-rpl27

Genome · 53953-54283 · 331 bp

Back to Nannochloropsis

Pi 0.185

MarkerSeek score 68.20

Primer available yes

Label thiG-rpl27

Feature Metadata

label_name: thiG-rpl27
feature_type: igs
region: Genome
length_bp: 331
variable_sites: 77
pi: 0.185
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG-rpl27_p1	GGCCTTTCTGCTGATGCTAT	ATCGTGTTATTCGATCGCGT	50.0	45.0	58.0	57.9	1451-1657 bp	0.75	80.9
thiG-rpl27_p2	GCGGACCCTAAAGGCATTAA	ATCGTGTTATTCGATCGCGT	50.0	45.0	58.0	57.9	1274-1480 bp	0.5	68.0
thiG-rpl27_p3	GGCCTTTCTGCTGATGCTAT	GCGACAATGTTGGTATTGGC	50.0	50.0	58.0	58.1	1199 bp	0.25	49.1
thiG-rpl27_p4	GCGGACCCTAAAGGCATTAA	GCGACAATGTTGGTATTGGC	50.0	50.0	58.0	58.1	1022 bp	0.25	48.9
thiG-rpl27_p5	AGCATCTGCACCAATTTCCA	GCGACAATGTTGGTATTGGC	45.0	50.0	58.1	58.1	1161 bp	0.25	48.7