Hotspot

P417_r006-psaC

Genome · 52527-52687 · 161 bp

Back to Nannochloropsis

Pi 0.12
MarkerSeek score 81.40
Primer available yes
Label P417_r006-psaC

Feature Metadata

label_name
P417_r006-psaC
feature_type
igs
region
Genome
length_bp
161
variable_sites
35
pi
0.12
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
P417_r006-psaC_p1 GAAGGTAGCTCAATGCCTGG TGCAACCAATACACGTGTCA 55.0 45.0 58.3 58.0 221-226 bp 1.0 87.9
P417_r006-psaC_p2 GGAAGGTAGCTCAATGCCTG TGCAACCAATACACGTGTCA 55.0 45.0 58.3 58.0 222-227 bp 1.0 87.9
P417_r006-psaC_p3 CGAACTCAGATGTGAAACGC TGCAACCAATACACGTGTCA 50.0 45.0 57.5 58.0 277-282 bp 1.0 86.0
P417_r006-psaC_p4 GAAGGTAGCTCAATGCCTGG GTGCAACCAATACACGTGTC 55.0 50.0 58.3 57.7 222-227 bp 1.0 85.8
P417_r006-psaC_p5 GGAAGGTAGCTCAATGCCTG GTGCAACCAATACACGTGTC 55.0 50.0 58.3 57.7 223-228 bp 1.0 85.7