Hotspot

rpl32-psbV

Genome · 44835-45471 · 637 bp

Back to Nannochloropsis

Pi 0.105

MarkerSeek score 80.20

Primer available yes

Label rpl32-psbV

Feature Metadata

label_name: rpl32-psbV
feature_type: igs
region: Genome
length_bp: 637
variable_sites: 118
pi: 0.105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-psbV_p1	GGATGTAGCGCAGTTTGGTA	TCAGAAAAATGGGGTGGTGG	50.0	50.0	58.0	58.0	920-924 bp	1.0	88.0
rpl32-psbV_p2	TAACAACACGGGATGTAGCG	TCAGAAAAATGGGGTGGTGG	50.0	50.0	58.0	58.0	930-934 bp	0.75	77.1
rpl32-psbV_p3	TAACAACACGGGATGTAGCG	ACCCGGAAGCTCTTAGTCTT	50.0	50.0	58.0	58.1	1145-1149 bp	0.75	76.3
rpl32-psbV_p4	TAACAACACGGGATGTAGCG	ATCAAACCTCGTTTTGCCCT	50.0	45.0	58.0	58.0	1371-1372 bp	0.5	60.5
rpl32-psbV_p5	GGATGTAGCGCAGTTTGGTA	ATCAAACCTCGTTTTGCCCT	50.0	45.0	58.0	58.0	1361-1362 bp	0.5	60.3