Hotspot

P417_t011-P417_t012

Genome · 44413-44584 · 172 bp

Back to Nannochloropsis

Pi 0.151
MarkerSeek score 78.60
Primer available yes
Label P417_t011-P417_t012

Feature Metadata

label_name
P417_t011-P417_t012
feature_type
igs
region
Genome
length_bp
172
variable_sites
45
pi
0.151
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
P417_t011-P417_t012_p1 CGGAAACCATTACTTTATCCT TACCAAACTGCGCTACATCC 38.1 50.0 53.3 58.0 226-230 bp 1.0 49.7
P417_t011-P417_t012_p2 CGGAAACCATTACTTTATCCTT TACCAAACTGCGCTACATCC 36.4 50.0 54.2 58.0 226-230 bp 1.0 49.7
P417_t011-P417_t012_p3 CGGAAACCATTACTTTATCCT CTGTGACATTCTGCTCCCAA 38.1 50.0 53.3 57.8 257-261 bp 1.0 49.5
P417_t011-P417_t012_p4 CGGAAACCATTACTTTATCCT AACCTGTGACATTCTGCTCC 38.1 50.0 53.3 57.8 260-264 bp 1.0 49.5
P417_t011-P417_t012_p5 CGGAAACCATTACTTTATCCTT CTGTGACATTCTGCTCCCAA 36.4 50.0 54.2 57.8 257-261 bp 1.0 49.5