primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
P417_t002-P417_t003_p1	P417_t002-P417_t003	1	GCTACCAATTTCGCCACATC	TGGAGACCTACGTTCTACCA	20	20	50.0	50.0	57.5	57.1	0.0	0.0	1.360335	5895	6546	707-732	1.000000	79.7
P417_t002-P417_t003_p2	P417_t002-P417_t003	2	TACCAATTTCGCCACATCCG	TGGAGACCTACGTTCTACCA	20	20	50.0	50.0	58.6	57.1	0.0	0.0	1.510575	5895	6546	705-730	1.000000	78.5
P417_t002-P417_t003_p3	P417_t002-P417_t003	3	GCTACCAATTTCGCCACATC	TGGAGACCTACGTTCTACCAA	20	21	50.0	47.6	57.5	57.8	0.0	0.0	1.634414	5895	6546	707-732	1.000000	77.5
P417_t002-P417_t003_p4	P417_t002-P417_t003	4	GCTACCAATTTCGCCACATC	TTGGAGACCTACGTTCTACCA	20	21	50.0	47.6	57.5	57.8	0.0	0.0	1.634414	5895	6546	708-733	1.000000	77.5
P417_t002-P417_t003_p5	P417_t002-P417_t003	5	GGCTACCAATTTCGCCACAT	TGGAGACCTACGTTCTACCA	20	20	50.0	50.0	58.9	57.1	0.0	0.0	1.782157	5895	6546	708-733	1.000000	76.3
rpoA_p1	rpoA	1	CCCACCACCAGAAACATCAA	ACATAATGGGTGTCGTGCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030220	20330	21652	2136-2136	0.750000	75.9
rpoA_p2	rpoA	2	CCCACCACCAGAAACATCAA	AAATGGACCTGGAAACGGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.100407	20330	21652	2229-2229	0.500000	63.7
rpoA_p3	rpoA	3	CCCACCACCAGAAACATCAA	CGACGTCACATACAGGGTTT	20	20	50.0	50.0	58.0	57.9	0.0	17.2	0.080591	20330	21652	2657-2657	0.500000	63.6
rpoA_p4	rpoA	4	CCCACCACCAGAAACATCAA	GGCAGGAAGTAGCGGTTTTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.109581	20330	21652	2349-2349	0.500000	63.6
rpoA_p5	rpoA	5	CCCACCACCAGAAACATCAA	ACGGTCGTGAAATTGCCATA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.108114	20330	21652	2215-2215	0.500000	61.8
ycf34_p1	ycf34	1	GGACTGAAGATCCGTGTGTC	CTGTTGCGTTTGCCCTATTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021077	34784	35035	935-966	1.000000	91.8
ycf34_p2	ycf34	2	GCAATGGACTGAAGATCCGT	CTGTTGCGTTTGCCCTATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048585	34784	35035	940-971	1.000000	91.6
ycf34_p3	ycf34	3	GGACTGAAGATCCGTGTGTC	CCCATACTGTTTGCAGGTGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.092631	34784	35035	1174-1205	1.000000	90.5
ycf34_p4	ycf34	4	GGACTGAAGATCCGTGTGTC	TGCACCGTGTAATGCGATAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.013871	34784	35035	1274-1305	0.750000	80.6
ycf34_p5	ycf34	5	GCAATGGACTGAAGATCCGT	TGCACCGTGTAATGCGATAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.041378	34784	35035	1279-1310	0.750000	80.3
chlI_p1	chlI	1	CCATGGGTAGAACCACTTCG	CGGTGATTTCTTTGCAGCTG	20	20	55.0	50.0	58.0	58.0	2.8	10.1	0.051037	37972	39207	2350-2354	0.750000	76.1
chlI_p2	chlI	2	TGGGATTTACGTGCTCCATG	CGGTGATTTCTTTGCAGCTG	20	20	50.0	50.0	58.0	58.0	13.0	10.1	0.063819	37972	39207	2365-2369	0.750000	75.9
chlI_p3	chlI	3	GATTTACGTGCTCCATGGGT	CGGTGATTTCTTTGCAGCTG	20	20	50.0	50.0	58.0	58.0	13.0	10.1	0.065249	37972	39207	2362-2366	0.750000	75.9
chlI_p4	chlI	4	CCATGGGTAGAACCACTTCG	GTGCCAAGATGAAAATCGCC	20	20	55.0	50.0	58.0	58.1	2.8	0.0	0.110859	37972	39207	2372-2381	0.750000	75.4
chlI_p5	chlI	5	CCATGGGTAGAACCACTTCG	GCCAAGATGAAAATCGCCAC	20	20	55.0	50.0	58.0	58.1	2.8	0.0	0.110859	37972	39207	2370-2373	0.500000	62.6
chlB_p1	chlB	1	AGACGTCTAGTGGAACGACT	ACACGCGCCTAGTTTTTACA	20	20	50.0	45.0	57.8	58.1	18.5	5.7	0.313485	41249	42769	2325-2327	1.000000	85.6
chlB_p2	chlB	2	AGACGTCTAGTGGAACGACT	GCCACAAATTGCTCGTGATC	20	20	50.0	50.0	57.8	58.1	18.5	0.0	0.281717	41249	42769	2193-2194	0.750000	74.5
chlB_p3	chlB	3	CCGATTGCTCTACCACTGAG	GCCACAAATTGCTCGTGATC	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.315670	41249	42769	2435-2436	0.750000	74.5
chlB_p4	chlB	4	AGACGTCTAGTGGAACGACT	AAAGCCACAAATTGCTCGTG	20	20	50.0	45.0	57.8	57.9	18.5	0.0	0.320550	41249	42769	2196-2197	0.750000	74.2
chlB_p5	chlB	5	AGACGTCTAGTGGAACGACT	ATTAGGCTCCGTGCTATTGC	20	20	50.0	50.0	57.8	58.1	18.5	0.0	0.297719	41249	42769	2039-2040	0.500000	61.1
P417_t011-P417_t012_p1	P417_t011-P417_t012	1	CGGAAACCATTACTTTATCCT	TACCAAACTGCGCTACATCC	21	20	38.1	50.0	53.3	58.0	0.0	9.7	5.711672	44413	44584	226-230	1.000000	49.7
P417_t011-P417_t012_p2	P417_t011-P417_t012	2	CGGAAACCATTACTTTATCCTT	TACCAAACTGCGCTACATCC	22	20	36.4	50.0	54.2	58.0	0.0	9.7	5.851702	44413	44584	226-230	1.000000	49.7
P417_t011-P417_t012_p3	P417_t011-P417_t012	3	CGGAAACCATTACTTTATCCT	CTGTGACATTCTGCTCCCAA	21	20	38.1	50.0	53.3	57.8	0.0	0.0	5.882724	44413	44584	257-261	1.000000	49.5
P417_t011-P417_t012_p4	P417_t011-P417_t012	4	CGGAAACCATTACTTTATCCT	AACCTGTGACATTCTGCTCC	21	20	38.1	50.0	53.3	57.8	0.0	0.0	5.884258	44413	44584	260-264	1.000000	49.5
P417_t011-P417_t012_p5	P417_t011-P417_t012	5	CGGAAACCATTACTTTATCCTT	CTGTGACATTCTGCTCCCAA	22	20	36.4	50.0	54.2	57.8	0.0	0.0	6.022754	44413	44584	257-261	1.000000	49.5
rpl32-psbV_p1	rpl32-psbV	1	GGATGTAGCGCAGTTTGGTA	TCAGAAAAATGGGGTGGTGG	20	20	50.0	50.0	58.0	58.0	11.5	0.0	0.026784	44835	45471	920-924	1.000000	88.0
rpl32-psbV_p2	rpl32-psbV	2	TAACAACACGGGATGTAGCG	TCAGAAAAATGGGGTGGTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009194	44835	45471	930-934	0.750000	77.1
rpl32-psbV_p3	rpl32-psbV	3	TAACAACACGGGATGTAGCG	ACCCGGAAGCTCTTAGTCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.7	0.060972	44835	45471	1145-1149	0.750000	76.3
rpl32-psbV_p4	rpl32-psbV	4	TAACAACACGGGATGTAGCG	ATCAAACCTCGTTTTGCCCT	20	20	50.0	45.0	58.0	58.0	0.0	3.1	0.005575	44835	45471	1371-1372	0.500000	60.5
rpl32-psbV_p5	rpl32-psbV	5	GGATGTAGCGCAGTTTGGTA	ATCAAACCTCGTTTTGCCCT	20	20	50.0	45.0	58.0	58.0	11.5	3.1	0.023166	44835	45471	1361-1362	0.500000	60.3
clpC-I_p1	clpC-I	1	CCACCACCCCATTTTTCTGA	GTTTCCATCCGTTATCCCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.031186	46004	47347	2262-2295	1.000000	89.3
clpC-I_p2	clpC-I	2	CCACCACCCCATTTTTCTGA	TTCTCTTCAGGCAGAGGTGA	20	20	50.0	50.0	58.0	58.0	0.0	1.0	0.023699	46004	47347	2363-2371	0.750000	76.6
clpC-I_p3	clpC-I	3	AGGGCAAAACGAGGTTTGAT	TTCTCTTCAGGCAGAGGTGA	20	20	45.0	50.0	58.0	58.0	16.8	1.0	0.020080	46004	47347	1930-1930	0.500000	59.9
clpC-I_p4	clpC-I	4	AGGGCAAAACGAGGTTTGAT	GTTTCCATCCGTTATCCCCC	20	20	45.0	55.0	58.0	58.0	16.8	0.0	0.027568	46004	47347	1854-1854	0.500000	59.8
clpC-I_p5	clpC-I	5	AGGGCAAAACGAGGTTTGAT	GGCATATGGGGTTTTAGCGA	20	20	45.0	50.0	58.0	58.0	16.8	4.6	0.028964	46004	47347	1876-1876	0.500000	59.8
P417_r006-psaC_p1	P417_r006-psaC	1	GAAGGTAGCTCAATGCCTGG	TGCAACCAATACACGTGTCA	20	20	55.0	45.0	58.3	58.0	0.0	27.2	0.371905	52527	52687	221-226	1.000000	87.9
P417_r006-psaC_p2	P417_r006-psaC	2	GGAAGGTAGCTCAATGCCTG	TGCAACCAATACACGTGTCA	20	20	55.0	45.0	58.3	58.0	0.0	27.2	0.371905	52527	52687	222-227	1.000000	87.9
P417_r006-psaC_p3	P417_r006-psaC	3	CGAACTCAGATGTGAAACGC	TGCAACCAATACACGTGTCA	20	20	50.0	45.0	57.5	58.0	0.0	27.2	0.539070	52527	52687	277-282	1.000000	86.0
P417_r006-psaC_p4	P417_r006-psaC	4	GAAGGTAGCTCAATGCCTGG	GTGCAACCAATACACGTGTC	20	20	55.0	50.0	58.3	57.7	0.0	27.2	0.636729	52527	52687	222-227	1.000000	85.8
P417_r006-psaC_p5	P417_r006-psaC	5	GGAAGGTAGCTCAATGCCTG	GTGCAACCAATACACGTGTC	20	20	55.0	50.0	58.3	57.7	0.0	27.2	0.636729	52527	52687	223-228	1.000000	85.7
thiG-rpl27_p1	thiG-rpl27	1	GGCCTTTCTGCTGATGCTAT	ATCGTGTTATTCGATCGCGT	20	20	50.0	45.0	58.0	57.9	0.0	11.1	0.136894	53953	54283	1451-1657	0.750000	80.9
thiG-rpl27_p2	thiG-rpl27	2	GCGGACCCTAAAGGCATTAA	ATCGTGTTATTCGATCGCGT	20	20	50.0	45.0	58.0	57.9	0.0	11.1	0.162350	53953	54283	1274-1480	0.500000	68.0
thiG-rpl27_p3	thiG-rpl27	3	GGCCTTTCTGCTGATGCTAT	GCGACAATGTTGGTATTGGC	20	20	50.0	50.0	58.0	58.1	0.0	2.4	0.113480	53953	54283	1199-1199	0.250000	49.1
thiG-rpl27_p4	thiG-rpl27	4	GCGGACCCTAAAGGCATTAA	GCGACAATGTTGGTATTGGC	20	20	50.0	50.0	58.0	58.1	0.0	2.4	0.138937	53953	54283	1022-1022	0.250000	48.9
thiG-rpl27_p5	thiG-rpl27	5	AGCATCTGCACCAATTTCCA	GCGACAATGTTGGTATTGGC	20	20	45.0	50.0	58.1	58.1	4.8	2.4	0.164447	53953	54283	1161-1161	0.250000	48.7
ycf54-secA_p1	ycf54-secA	1	CTCAGGCGAAGGAAGAATCC	GATGCAGGAAGAGTGGCTAC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.106510	55461	57755	2731-2783	0.500000	65.3
ycf54-secA_p2	ycf54-secA	2	CTCAGGCGAAGGAAGAATCC	GTACACCTTTGCCGCTAAGA	20	20	55.0	50.0	58.1	57.9	0.0	15.1	0.142205	55461	57755	2812-2812	0.250000	48.9
ycf54-secA_p3	ycf54-secA	3	CTCAGGCGAAGGAAGAATCC	TTGCCGCTAAGAGCTTGAAA	20	20	55.0	45.0	58.1	58.1	0.0	0.0	0.162379	55461	57755	2804-2804	0.250000	48.7
ycf54-secA_p4	ycf54-secA	4	CTCAGGCGAAGGAAGAATCC	TTTGCCGCTAAGAGCTTGAA	20	20	55.0	45.0	58.1	58.1	0.0	0.0	0.162379	55461	57755	2805-2805	0.250000	48.7
ycf54-secA_p5	ycf54-secA	5	CTCAGGCGAAGGAAGAATCC	CGAGCCCTCCAAGAATTTGA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.167115	55461	57755	2710-2710	0.250000	48.7
rbcS-psbA_p1	rbcS-psbA	1	TCCTCCTGGAGAACGTTACG	GTTGCATTAAACGCTCCTGC	20	20	55.0	50.0	58.8	58.4	25.2	0.0	1.210952	92991	93369	423-760	1.000000	82.3
rbcS-psbA_p2	rbcS-psbA	2	TCCTCCTGGAGAACGTTACG	TTGCATTAAACGCTCCTGCT	20	20	55.0	45.0	58.8	58.5	25.2	0.0	1.295067	92991	93369	422-759	1.000000	81.6
rbcS-psbA_p3	rbcS-psbA	3	CCTCCTGGAGAACGTTACGT	GTTGCATTAAACGCTCCTGC	20	20	55.0	50.0	59.1	58.4	25.2	0.0	1.495698	92991	93369	422-759	1.000000	80.0
rbcS-psbA_p4	rbcS-psbA	4	CCTCCTGGAGAACGTTACGT	TTGCATTAAACGCTCCTGCT	20	20	55.0	45.0	59.1	58.5	25.2	0.0	1.579812	92991	93369	421-758	1.000000	79.4
rbcS-psbA_p5	rbcS-psbA	5	TCCTCCTGGAGAACGTTACG	CGCTCCTGCTGTTAATGCTT	20	20	55.0	50.0	58.8	58.9	25.2	5.3	1.738772	92991	93369	412-749	1.000000	78.1
chlN_p1	chlN	1	AGCGCTCCCATAAGCTAGTA	TCGCGAAAAAGCTAGGACTC	20	20	50.0	50.0	58.0	58.0	2.6	6.2	0.011032	94673	95998	2013-2019	0.750000	75.6
chlN_p2	chlN	2	CACCGAACCAACCGATGTAT	TCGCGAAAAAGCTAGGACTC	20	20	50.0	50.0	58.0	58.0	0.0	6.2	0.026372	94673	95998	2067-2073	0.750000	75.4
chlN_p3	chlN	3	GCGCTCCCATAAGCTAGTAC	TCGCGAAAAAGCTAGGACTC	20	20	55.0	50.0	57.9	58.0	0.0	6.2	0.075244	94673	95998	2012-2018	0.750000	75.1
chlN_p4	chlN	4	CATTCACGTCCCATGTAGCA	TCGCGAAAAAGCTAGGACTC	20	20	50.0	50.0	58.0	58.0	0.0	6.2	0.030595	94673	95998	2356-2362	0.750000	74.7
chlN_p5	chlN	5	CCCATTCACGTCCCATGTAG	TCGCGAAAAAGCTAGGACTC	20	20	55.0	50.0	58.0	58.0	0.0	6.2	0.054271	94673	95998	2358-2364	0.750000	74.5
rps16-P417_t022_p1	rps16-P417_t022	1	TCCAAAAGCAATTCCACCGA	TATCGCCAGGTTGACATTGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.048664	99715	100553	1950-1953	1.000000	87.8
rps16-P417_t022_p2	rps16-P417_t022	2	CTACCCCATACCACCATTGC	TATCGCCAGGTTGACATTGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.055701	99715	100553	1875-1878	1.000000	87.8
rps16-P417_t022_p3	rps16-P417_t022	3	GAGCACCTTGTGATAAGCGA	TATCGCCAGGTTGACATTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047103	99715	100553	1637-1640	0.500000	64.3
rps16-P417_t022_p4	rps16-P417_t022	4	GAGCACCTTGTGATAAGCGA	AGTTGAATGCTATCGCCAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051103	99715	100553	1647-1650	0.500000	64.2
rps16-P417_t022_p5	rps16-P417_t022	5	TCCAAAAGCAATTCCACCGA	AGTTGAATGCTATCGCCAGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.052665	99715	100553	1960-1963	0.500000	63.9
psaI-psbJ_p1	psaI-psbJ	1	GAAGGGGTTTTACCTGGTGG	GCTTGTAGCCTTTGTTGCAG	20	20	55.0	50.0	58.1	57.9	10.2	0.0	0.136854	103911	103965	754-755	0.750000	77.2
psaI-psbJ_p2	psaI-psbJ	2	GAAGGGGTTTTACCTGGTGG	TGTAGCCTTTGTTGCAGGTT	20	20	55.0	45.0	58.1	57.9	10.2	0.0	0.146658	103911	103965	751-752	0.750000	77.1
psaI-psbJ_p3	psaI-psbJ	3	GAAGGGGTTTTACCTGGTGG	TTGTAGCCTTTGTTGCAGGT	20	20	55.0	45.0	58.1	57.9	10.2	0.0	0.146658	103911	103965	752-753	0.750000	77.1
psaI-psbJ_p4	psaI-psbJ	4	GAAGGGGTTTTACCTGGTGG	ACGTTTCGTTGGTTAGCAGT	20	20	55.0	45.0	58.1	58.1	10.2	0.0	0.136728	103911	103965	1088-1090	0.750000	75.2
psaI-psbJ_p5	psaI-psbJ	5	GAAGGGGTTTTACCTGGTGG	GAACGCCCATTCTCAGACAT	20	20	55.0	50.0	58.1	58.0	10.2	0.0	0.110598	103911	103965	1366-1368	0.750000	74.3
ycf49-psaL_p1	ycf49-psaL	1	ACTTTGTGCCCCAAATCCAT	GCAGAACGTAATGGGCCATA	20	20	45.0	50.0	58.0	58.0	0.0	21.5	0.066768	105732	106263	1231-1234	0.500000	62.3
ycf49-psaL_p2	ycf49-psaL	2	ACTTTGTGCCCCAAATCCAT	AAGGCTATACCAACTGCAGC	20	20	45.0	50.0	58.0	58.0	0.0	15.3	0.072684	105732	106263	1519-1522	0.500000	62.3
ycf49-psaL_p3	ycf49-psaL	3	ACTTTGTGCCCCAAATCCAT	TGGTGTTAGTCCTGGTCGAT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.095540	105732	106263	1148-1151	0.500000	62.2
ycf49-psaL_p4	ycf49-psaL	4	GTTTTCGGACCGGACTATCC	GCAGAACGTAATGGGCCATA	20	20	55.0	50.0	58.1	58.0	15.2	21.5	0.106300	105732	106263	1463-1463	0.250000	49.1
ycf49-psaL_p5	ycf49-psaL	5	GTTTTCGGACCGGACTATCC	AAGGCTATACCAACTGCAGC	20	20	55.0	50.0	58.1	58.0	15.2	15.3	0.112217	105732	106263	1751-1751	0.250000	49.1