Hotspot

rps16

Genome · 30754-31922 · 1169 bp

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Pi 0.000855

MarkerSeek score 59.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1169
variable_sites: 1
pi: 0.000855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATAGAACAAGAGAGCGGGGG	TGAATCAATGACTATGCACGA	55.0	38.1	58.9	55.4	1327-1328 bp	1.0	44.5
rps16_p2	AATAGAACAAGAGAGCGGGG	TGAATCAATGACTATGCACGA	50.0	38.1	57.0	55.4	1328-1329 bp	1.0	43.6
rps16_p3	ATAGAACAAGAGAGCGGGGG	TCAATGACTATGCACGATTTCA	55.0	36.4	58.9	56.2	1323-1324 bp	1.0	42.5
rps16_p4	ATAGAACAAGAGAGCGGGGG	TTGAATCAATGACTATGCACGA	55.0	36.4	58.9	56.2	1328-1329 bp	1.0	42.5
rps16_p5	GAATAGAACAAGAGAGCGGGG	TGAATCAATGACTATGCACGA	52.4	38.1	58.2	55.4	1329-1330 bp	1.0	42.1