Hotspot

rps11

Genome · 7600-8016 · 417 bp

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Pi 0

MarkerSeek score 24.90

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: Genome
length_bp: 417
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TCCGTTTTTGACAAGAAATGGA	TTTCTCTTGAAATCTCTTTCTTT	36.4	26.1	56.7	52.2	523 bp	1.0	40.0
rps11_p2	TCCGTTTTTGACAAGAAATGGA	TCTCTTGAAATCTCTTTCTTTAATGT	36.4	26.9	56.7	55.2	521 bp	1.0	40.0
rps11_p3	TCCGTTTTTGACAAGAAATGGA	ATTTCTCTTGAAATCTCTTTCTT	36.4	26.1	56.7	52.0	524 bp	1.0	40.0
rps11_p4	TCCGTTTTTGACAAGAAATGGA	ATTTCTCTTGAAATCTCTTTCTTT	36.4	25.0	56.7	52.9	524 bp	1.0	40.0
rps11_p5	TCCGTTTTTGACAAGAAATGGA	CTCTTGAAATCTCTTTCTTTAATGT	36.4	28.0	56.7	53.8	520 bp	1.0	40.0