Hotspot

trnS-GCU-trnG-UCC

Genome · 34230-35032 · 803 bp

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Pi 0
MarkerSeek score 61.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
Genome
length_bp
803
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAGTCGC 50.0 55.0 58.0 57.9 862-883 bp 1.0 81.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAGTCGC 50.0 55.0 58.0 57.9 861-882 bp 1.0 81.5
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA GGATCCATAGAACGAGTCGC 50.0 55.0 58.2 57.9 915-936 bp 1.0 80.2
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA CGAGTCGCACTTTTACCACT 50.0 50.0 58.0 58.2 850-871 bp 1.0 80.2
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC CGAGTCGCACTTTTACCACT 50.0 50.0 58.0 58.2 849-870 bp 1.0 80.1