Hotspot

trnQ-UUG-psbK

Genome · 32475-33200 · 726 bp

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Pi 0.00138
MarkerSeek score 59.50
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
Genome
length_bp
726
variable_sites
1
pi
0.00138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CGAATAACGGGACCAAAACC CGTAGGCTTCAGGCAATTTC 50.0 50.0 57.1 57.2 865 bp 1.0 66.2
trnQ-UUG-psbK_p2 CGAATAACGGGACCAAAACC GTAGGCTTCAGGCAATTTCG 50.0 50.0 57.1 57.2 864 bp 1.0 66.2
trnQ-UUG-psbK_p3 CGAATAACGGGACCAAAACC TAGGCTTCAGGCAATTTCGC 50.0 50.0 57.1 58.9 863 bp 1.0 65.6
trnQ-UUG-psbK_p4 CGAATAACGGGACCAAAACC GCGTAGGCTTCAGGCAATTT 50.0 50.0 57.1 59.2 866 bp 1.0 63.3
trnQ-UUG-psbK_p5 GAATAACGGGACCAAAACCC GTAGGCTTCAGGCAATTTCG 50.0 50.0 56.7 57.2 863 bp 1.0 62.9