Hotspot

rps11-rpoA

Genome · 8017-8095 · 79 bp

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Pi 0.0127

MarkerSeek score 59.80

Primer available yes

Label rps11-rpoA

Feature Metadata

label_name: rps11-rpoA
feature_type: igs
region: Genome
length_bp: 79
variable_sites: 1
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpoA_p1	AGAGATGCGGCATTAAGAGC	ACTTCCACTGTAGTGTCCGA	50.0	50.0	58.1	58.0	242 bp	1.0	79.3
rps11-rpoA_p2	AGAGATGCGGCATTAAGAGC	TTCCACTGTAGTGTCCGAGT	50.0	50.0	58.1	58.0	240 bp	1.0	79.3
rps11-rpoA_p3	GAGATGCGGCATTAAGAGCT	ACTTCCACTGTAGTGTCCGA	50.0	50.0	58.1	58.0	241 bp	1.0	79.3
rps11-rpoA_p4	GAGATGCGGCATTAAGAGCT	TTCCACTGTAGTGTCCGAGT	50.0	50.0	58.1	58.0	239 bp	1.0	79.3
rps11-rpoA_p5	GAGATGCGGCATTAAGAGCT	CACTTCCACTGTAGTGTCCG	50.0	55.0	58.1	57.9	242 bp	1.0	78.8