primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	rps11	1	TCCGTTTTTGACAAGAAATGGA	TTTCTCTTGAAATCTCTTTCTTT	22	23	36.4	26.1	56.7	52.2	20.9	0.4	12.031765	7600	8016	523-523	1.000000	40.0
rps11_p2	rps11	2	TCCGTTTTTGACAAGAAATGGA	TCTCTTGAAATCTCTTTCTTTAATGT	22	26	36.4	26.9	56.7	55.2	20.9	0.4	12.099372	7600	8016	521-521	1.000000	40.0
rps11_p3	rps11	3	TCCGTTTTTGACAAGAAATGGA	ATTTCTCTTGAAATCTCTTTCTT	22	23	36.4	26.1	56.7	52.0	20.9	0.0	12.250819	7600	8016	524-524	1.000000	40.0
rps11_p4	rps11	4	TCCGTTTTTGACAAGAAATGGA	ATTTCTCTTGAAATCTCTTTCTTT	22	24	36.4	25.0	56.7	52.9	20.9	0.0	12.397082	7600	8016	524-524	1.000000	40.0
rps11_p5	rps11	5	TCCGTTTTTGACAAGAAATGGA	CTCTTGAAATCTCTTTCTTTAATGT	22	25	36.4	28.0	56.7	53.8	20.9	0.4	12.419774	7600	8016	520-520	1.000000	40.0
rps11-rpoA_p1	rps11-rpoA	1	AGAGATGCGGCATTAAGAGC	ACTTCCACTGTAGTGTCCGA	20	20	50.0	50.0	58.1	58.0	17.8	10.8	0.132557	8017	8095	242-242	1.000000	79.3
rps11-rpoA_p2	rps11-rpoA	2	AGAGATGCGGCATTAAGAGC	TTCCACTGTAGTGTCCGAGT	20	20	50.0	50.0	58.1	58.0	17.8	10.8	0.132557	8017	8095	240-240	1.000000	79.3
rps11-rpoA_p3	rps11-rpoA	3	GAGATGCGGCATTAAGAGCT	ACTTCCACTGTAGTGTCCGA	20	20	50.0	50.0	58.1	58.0	17.8	10.8	0.132557	8017	8095	241-241	1.000000	79.3
rps11-rpoA_p4	rps11-rpoA	4	GAGATGCGGCATTAAGAGCT	TTCCACTGTAGTGTCCGAGT	20	20	50.0	50.0	58.1	58.0	17.8	10.8	0.132557	8017	8095	239-239	1.000000	79.3
rps11-rpoA_p5	rps11-rpoA	5	GAGATGCGGCATTAAGAGCT	CACTTCCACTGTAGTGTCCG	20	20	50.0	55.0	58.1	57.9	17.8	10.8	0.189671	8017	8095	242-242	1.000000	78.8
psbB-rps12_p1	psbB-rps12	1	TGGACAGAAAGTAAACGGCC	AGAGCGGGAGATTTTGTGAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.213718	14617	16936	2458-2459	1.000000	78.3
psbB-rps12_p2	psbB-rps12	2	AAACGGCCGGGATCATTTAA	AGAGCGGGAGATTTTGTGAC	20	20	45.0	50.0	57.9	57.9	31.8	0.0	0.232380	14617	16936	2446-2447	1.000000	78.2
psbB-rps12_p3	psbB-rps12	3	TGGACAGAAAGTAAACGGCC	ACCCTAGTACAGGTTCCTCG	20	20	50.0	55.0	58.1	57.9	0.0	9.6	0.253952	14617	16936	2497-2498	1.000000	78.0
psbB-rps12_p4	psbB-rps12	4	AAACGGCCGGGATCATTTAA	ACCCTAGTACAGGTTCCTCG	20	20	45.0	55.0	57.9	57.9	31.8	9.6	0.272615	14617	16936	2485-2486	1.000000	77.9
psbB-rps12_p5	psbB-rps12	5	TGGACAGAAAGTAAACGGCC	GAGCGGGAGATTTTGTGACA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.304703	14617	16936	2457-2458	1.000000	77.6
rps12_p1	rps12	1	AAAATTAGGATGATGCGTCA	AGAGCATATCTCCAGACACCT	20	21	35.0	47.6	53.0	57.7	11.8	0.0	6.370680	76289	76408	223-223	1.000000	40.7
rps12_p2	rps12	2	AAATTAGGATGATGCGTCAA	AGAGCATATCTCCAGACACCT	20	21	35.0	47.6	53.0	57.7	13.7	0.0	6.370680	76289	76408	222-222	1.000000	40.7
rps12_p3	rps12	3	AAAATTAGGATGATGCGTCAA	AGAGCATATCTCCAGACACCT	21	21	33.3	47.6	53.9	57.7	13.7	0.0	6.455338	76289	76408	223-223	1.000000	40.7
rps12_p4	rps12	4	ACTACTGAAACTGACAAAGTCT	AGAGCATATCTCCAGACACCT	22	21	36.4	47.6	55.0	57.7	0.0	0.0	6.329728	76289	76408	285-285	1.000000	40.6
rps12_p5	rps12	5	ACTACTGAAACTGACAAAGTCTT	AGAGCATATCTCCAGACACCT	23	21	34.8	47.6	55.8	57.7	0.0	0.0	6.576413	76289	76408	285-285	1.000000	40.6
petL-psbE_p1	petL-psbE	1	AACCGCTAGTAGAAAACCGA	TGACAAATAACCAACCCGCA	20	20	45.0	45.0	56.6	58.0	0.0	0.0	1.420951	21154	22649	1653-1653	1.000000	68.7
petL-psbE_p2	petL-psbE	2	ACCGCTAGTAGAAAACCGAA	TGACAAATAACCAACCCGCA	20	20	45.0	45.0	56.6	58.0	0.0	0.0	1.420951	21154	22649	1652-1652	1.000000	68.7
petL-psbE_p3	petL-psbE	3	AACCGCTAGTAGAAAACCGA	CAACCCGCAATGAATAGGGA	20	20	45.0	50.0	56.6	57.9	0.0	6.4	1.454946	21154	22649	1642-1642	1.000000	68.4
petL-psbE_p4	petL-psbE	4	ACCGCTAGTAGAAAACCGAA	CAACCCGCAATGAATAGGGA	20	20	45.0	50.0	56.6	57.9	0.0	6.4	1.454946	21154	22649	1641-1641	1.000000	68.4
petL-psbE_p5	petL-psbE	5	ACCGCTAGTAGAAAACCGAA	AGAACGTTCTCCTGTGCTTC	20	20	45.0	50.0	56.6	57.8	0.0	24.9	1.559955	21154	22649	1564-1564	1.000000	67.6
cemA_p1	cemA	1	AGGAAGAATACGAGTAGAAAGA	TGACAAATGAGATGAAGCTGAGT	22	23	36.4	39.1	53.8	58.1	0.0	0.0	9.317523	25696	26385	832-832	1.000000	40.1
cemA_p2	cemA	2	AGGAAGAATACGAGTAGAAAGAA	TGACAAATGAGATGAAGCTGAGT	23	23	34.8	39.1	54.6	58.1	0.0	0.0	9.508102	25696	26385	832-832	1.000000	40.1
cemA_p3	cemA	3	AGGAAGAATACGAGTAGAAAGAAA	TGACAAATGAGATGAAGCTGAGT	24	23	33.3	39.1	55.3	58.1	0.0	0.0	9.766306	25696	26385	832-832	1.000000	40.1
cemA_p4	cemA	4	AGGAAGAATACGAGTAGAAAGA	TGACAAATGAGATGAAGCTGAG	22	22	36.4	40.9	53.8	56.4	0.0	0.0	9.775856	25696	26385	832-832	1.000000	40.1
cemA_p5	cemA	5	AGGAAGAATACGAGTAGAAAGA	GACAAATGAGATGAAGCTGAGT	22	22	36.4	40.9	53.8	56.4	0.0	0.0	9.778050	25696	26385	831-831	1.000000	40.1
rps16_p1	rps16	1	ATAGAACAAGAGAGCGGGGG	TGAATCAATGACTATGCACGA	20	21	55.0	38.1	58.9	55.4	0.0	0.0	4.456056	30754	31922	1327-1328	1.000000	44.5
rps16_p2	rps16	2	AATAGAACAAGAGAGCGGGG	TGAATCAATGACTATGCACGA	20	21	50.0	38.1	57.0	55.4	0.0	0.0	4.570050	30754	31922	1328-1329	1.000000	43.6
rps16_p3	rps16	3	ATAGAACAAGAGAGCGGGGG	TCAATGACTATGCACGATTTCA	20	22	55.0	36.4	58.9	56.2	0.0	0.0	4.698269	30754	31922	1323-1324	1.000000	42.5
rps16_p4	rps16	4	ATAGAACAAGAGAGCGGGGG	TTGAATCAATGACTATGCACGA	20	22	55.0	36.4	58.9	56.2	0.0	0.0	4.698269	30754	31922	1328-1329	1.000000	42.5
rps16_p5	rps16	5	GAATAGAACAAGAGAGCGGGG	TGAATCAATGACTATGCACGA	21	21	52.4	38.1	58.2	55.4	0.0	0.0	4.752332	30754	31922	1329-1330	1.000000	42.1
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	CGAATAACGGGACCAAAACC	CGTAGGCTTCAGGCAATTTC	20	20	50.0	50.0	57.1	57.2	0.0	2.9	1.738631	32475	33200	865-865	1.000000	66.2
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	CGAATAACGGGACCAAAACC	GTAGGCTTCAGGCAATTTCG	20	20	50.0	50.0	57.1	57.2	0.0	2.9	1.738631	32475	33200	864-864	1.000000	66.2
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	CGAATAACGGGACCAAAACC	TAGGCTTCAGGCAATTTCGC	20	20	50.0	50.0	57.1	58.9	0.0	2.9	1.811174	32475	33200	863-863	1.000000	65.6
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	CGAATAACGGGACCAAAACC	GCGTAGGCTTCAGGCAATTT	20	20	50.0	50.0	57.1	59.2	0.0	2.9	2.094678	32475	33200	866-866	1.000000	63.3
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	GAATAACGGGACCAAAACCC	GTAGGCTTCAGGCAATTTCG	20	20	50.0	50.0	56.7	57.2	0.0	2.9	2.142853	32475	33200	863-863	1.000000	62.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAGTCGC	20	20	50.0	55.0	58.0	57.9	0.0	13.6	0.057865	34230	35032	862-883	1.000000	81.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	GGATCCATAGAACGAGTCGC	20	20	50.0	55.0	58.0	57.9	0.0	13.6	0.059253	34230	35032	861-882	1.000000	81.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	GGATCCATAGAACGAGTCGC	20	20	50.0	55.0	58.2	57.9	0.0	13.6	0.204630	34230	35032	915-936	1.000000	80.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	CGAGTCGCACTTTTACCACT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.225013	34230	35032	850-871	1.000000	80.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	CGAGTCGCACTTTTACCACT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.226401	34230	35032	849-870	1.000000	80.1
atpF_p1	atpF	1	TGCACGAATGGTTACCATGA	CCATAAGAGGAGATGCGATCA	20	21	45.0	47.6	57.5	57.1	18.5	0.0	2.431131	37923	39184	1377-1377	1.000000	60.6
atpF_p2	atpF	2	TGCACGAATGGTTACCATGA	TCCATAAGAGGAGATGCGATC	20	21	45.0	47.6	57.5	56.8	18.5	0.0	2.708776	37923	39184	1378-1378	1.000000	58.4
atpF_p3	atpF	3	ATCTGCACGAATGGTTACCA	CCATAAGAGGAGATGCGATCA	20	21	45.0	47.6	57.2	57.1	6.5	0.0	2.729597	37923	39184	1380-1380	1.000000	58.2
atpF_p4	atpF	4	CTGCACGAATGGTTACCATG	CCATAAGAGGAGATGCGATCA	20	21	50.0	47.6	57.2	57.1	18.5	0.0	2.780614	37923	39184	1378-1378	1.000000	57.8
atpF_p5	atpF	5	TGCACGAATGGTTACCATGA	CCATAAGAGGAGATGCGATCAT	20	22	45.0	45.5	57.5	57.5	18.5	0.0	2.950054	37923	39184	1377-1377	1.000000	56.5
atpH-atpI_p1	atpH-atpI	1	CGCAATCCCTTCTACAGCTT	GCTGCGGCTTATATAGGTGA	20	20	50.0	50.0	58.0	57.3	0.0	11.2	0.773474	39904	41326	1585-1585	1.000000	73.9
atpH-atpI_p2	atpH-atpI	2	CGCAATCCCTTCTACAGCTT	ACAAGCGGTATTCAAGCTCT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.800002	39904	41326	1621-1621	1.000000	73.6
atpH-atpI_p3	atpH-atpI	3	CGCAATCCCTTCTACAGCTT	GGTGAATCCATGGAAGGTCA	20	20	50.0	50.0	58.0	57.2	0.0	20.7	0.836783	39904	41326	1570-1570	1.000000	73.4
atpH-atpI_p4	atpH-atpI	4	AACAGAAGCGGCAGAAATCA	GCTGCGGCTTATATAGGTGA	20	20	45.0	50.0	58.1	57.3	1.4	11.2	0.847416	39904	41326	1495-1495	1.000000	73.3
atpH-atpI_p5	atpH-atpI	5	AACAGAAGCGGCAGAAATCA	ACAAGCGGTATTCAAGCTCT	20	20	45.0	45.0	58.1	57.2	1.4	0.0	0.873944	39904	41326	1531-1531	1.000000	73.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	73785	75302	1553-1568	1.000000	79.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	73785	75302	1561-1576	1.000000	79.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	73785	75302	1585-1600	1.000000	78.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	73785	75302	1593-1608	1.000000	78.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CCCATTGTGAATTCCAGGGT	20	20	55.0	50.0	57.9	57.8	0.0	2.3	0.369229	73785	75302	1646-1661	1.000000	77.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	20	20	55.0	50.0	58.0	57.9	10.3	3.3	0.074283	76367	77117	891-891	1.000000	79.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	20	20	50.0	50.0	58.1	57.9	0.0	3.3	0.123673	76367	77117	855-855	1.000000	79.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AGAATGGTCGGGATAGCTCA	TTGGATAGGATGGCCTTTGC	20	20	50.0	50.0	57.9	57.9	0.0	3.3	0.159882	76367	77117	900-900	1.000000	78.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	TTGGATAGGATGGCCTTTGC	20	20	55.0	50.0	57.9	57.9	2.5	3.3	0.208076	76367	77117	879-879	1.000000	78.5
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	AGTTCAAATCTGGTTCCCGG	TTGGATAGGATGGCCTTTGC	20	20	50.0	50.0	57.8	57.9	0.0	3.3	0.272150	76367	77117	843-843	1.000000	78.0