Hotspot

ccsA

SSC · 120834-121760 · 927 bp

Back to Najas

Pi 0.455
MarkerSeek score 83.30
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
927
variable_sites
402
pi
0.455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TAGACACGCTGCTCTTAGGA GCTTGGCCAATTACTCGAGA 50.0 50.0 57.9 58.0 1685 bp 0.5 58.8
ccsA_p2 TAAGCCGCCATGGTGAAATT GCTTGGCCAATTACTCGAGA 45.0 50.0 58.2 58.0 1707 bp 0.5 58.6
ccsA_p3 AAATTGGTAGACACGCTGCT GCTTGGCCAATTACTCGAGA 45.0 50.0 57.8 58.0 1692 bp 0.5 58.3
ccsA_p4 GGTAGACACGCTGCTCTTAG GCTTGGCCAATTACTCGAGA 55.0 50.0 57.8 58.0 1687 bp 0.5 58.2
ccsA_p5 GTAGACACGCTGCTCTTAGG GCTTGGCCAATTACTCGAGA 55.0 50.0 57.8 58.0 1686 bp 0.5 58.2