Hotspot

rpl22-rps19

LSC · 87435-88358 · 924 bp

Back to Najas

Pi 0.167
MarkerSeek score 76.80
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
924
variable_sites
108
pi
0.167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.70
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CAAGTCGGAAACCAAGTGGA GGAAGAGCCCCAATTGGTAG 50.0 55.0 58.0 57.9 1742-1959 bp 1.0 89.9
rpl22-rps19_p2 CTCCGTTGACTTCCGCTTTA CCGGATCGAAATGTTGGCTA 50.0 50.0 57.9 58.1 1613-1820 bp 1.0 89.9
rpl22-rps19_p3 CAAGTCGGAAACCAAGTGGA CCGGATCGAAATGTTGGCTA 50.0 50.0 58.0 58.1 1828-2045 bp 1.0 89.7
rpl22-rps19_p4 CAAGTCGGAAACCAAGTGGA GACAGGTGGGTAATGCTGAG 50.0 55.0 58.0 58.0 2090 bp 0.5 59.5
rpl22-rps19_p5 CTCCGTTGACTTCCGCTTTA GACAGGTGGGTAATGCTGAG 50.0 55.0 57.9 58.0 1865 bp 0.5 59.2