Hotspot

psbE-petL

LSC · 68400-69486 · 1087 bp

Back to Najas

Pi 0.113
MarkerSeek score 82.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1087
variable_sites
119
pi
0.113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.9 58.0 1717-1758 bp 1.0 88.2
psbE-petL_p2 ATACTCATTTGGCCGTGGAC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 1778-1819 bp 1.0 88.1
psbE-petL_p3 ACTGACGAATAACCAACCCG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.9 58.0 1730-1771 bp 1.0 87.9
psbE-petL_p4 TCCGCCATTTACCTTGCTTT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 58.1 58.0 2271-2316 bp 1.0 86.3
psbE-petL_p5 ATCCGCCATTTACCTTGCTT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 57.9 58.0 2272-2317 bp 1.0 85.8