Hotspot

petA-psbJ

LSC · 66770-67626 · 857 bp

Back to Najas

Pi 0.111
MarkerSeek score 86.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
857
variable_sites
91
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAAAAGGTTCAGT CATATTCCGGATTGGGCTCA 33.3 50.0 57.4 57.4 920-930 bp 1.0 50.9
petA-psbJ_p2 ACAGTTTGAAAAGGTTCAGT CATATTCCGGATTGGGCTCA 35.0 50.0 53.1 57.4 916-926 bp 1.0 50.9
petA-psbJ_p3 AGAAACAGTTTGAAAAGGTTCAGT ATTCCGGATTGGGCTCATCT 33.3 50.0 57.4 58.9 917-927 bp 1.0 50.9
petA-psbJ_p4 AGAAACAGTTTGAAAAGGTTCAGT TATTCCGGATTGGGCTCATC 33.3 50.0 57.4 57.1 918-928 bp 1.0 50.9
petA-psbJ_p5 AGAAACAGTTTGAAAAGGTTCAGT TCATATTCCGGATTGGGCTC 33.3 50.0 57.4 57.1 921-931 bp 1.0 50.9