Hotspot

tRNA-Phe(GAA)-tRNA-Val(UAC)

LSC · 52045-53671 · 1627 bp

Back to Najas

Pi 0.104
MarkerSeek score 81.90
Primer available yes
Label tRNA-Phe(GAA)-tRNA-Val(UAC)

Feature Metadata

label_name
tRNA-Phe(GAA)-tRNA-Val(UAC)
feature_type
igs
region
LSC
length_bp
1627
variable_sites
151
pi
0.104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Phe(GAA)-tRNA-Val(UAC)_p1 CCTCGTGTCACCAGTTCAAA ACAAACTGCATGTTGGGTCT 50.0 45.0 58.1 57.9 1700-1765 bp 1.0 90.2
tRNA-Phe(GAA)-tRNA-Val(UAC)_p2 ACATACACGGCCAAGGAATC ACAAACTGCATGTTGGGTCT 50.0 45.0 58.0 57.9 2035 bp 0.5 59.1
tRNA-Phe(GAA)-tRNA-Val(UAC)_p3 TATTTTTCGTCGGGGGTTCC ACAAACTGCATGTTGGGTCT 50.0 45.0 57.9 57.9 2177 bp 0.5 58.5
tRNA-Phe(GAA)-tRNA-Val(UAC)_p4 ACATACACGGCCAAGGAATC TTGTGGTGGAGCGATAGAGA 50.0 50.0 58.0 58.2 2276 bp 0.5 58.5
tRNA-Phe(GAA)-tRNA-Val(UAC)_p5 GTGCCTATTTGGTGCCTACA ACAAACTGCATGTTGGGTCT 50.0 45.0 57.9 57.9 2081 bp 0.5 58.4