Hotspot

tRNA-Glu(UUC)-tRNA-Thr(GGU)

LSC · 34526-35190 · 665 bp

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Pi 0.105
MarkerSeek score 83.40
Primer available yes
Label tRNA-Glu(UUC)-tRNA-Thr(GGU)

Feature Metadata

label_name
tRNA-Glu(UUC)-tRNA-Thr(GGU)
feature_type
igs
region
LSC
length_bp
665
variable_sites
64
pi
0.105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p1 TGACGAATTATTGGGCCGAG GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 919-956 bp 1.0 89.3
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p2 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 771-808 bp 1.0 89.3
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p3 TGACGAATTATTGGGCCGAG TACCGCTGAGTTAAAAGGGC 50.0 50.0 58.1 57.9 884-921 bp 1.0 88.7
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p4 TGACGAATTATTGGGCCGAG ATTTCGTGGAAAAAGCCCCT 50.0 45.0 58.1 58.0 986 bp 0.5 59.5
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p5 TGACGAATTATTGGGCCGAG CCCATGCCCCTAAATTTCGA 50.0 50.0 58.1 57.9 1651 bp 0.5 59.1